![]() Jawetz, Melnick, and Adelberg’s Medical Microbiology.Editors: Koneman E.W., Allen D.D., Dowell V.R. Colour Atlas and Textbook of Diagnostic Microbiology.Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC. Clinical Microbiology Procedure Handbook Vol.District Laboratory Practice in Tropical Countries – Part-2- Monica Cheesebrough- 2nd Edn Update.Weissfeld, 12th ed 2007, Publisher Elsevier. Bailey & Scott’s Diagnostic Microbiology.The reagent is stable for several months.Add distilled water to the 1-liter mark, and mix until the salt is fully dissolved.Weigh the sodium chloride, and transfer it to a leak-proof bottle premarked to hold 1 liter.The reagent is stable for several months.Ĭaution: Ethanol and methanol are highly flammable, therefore use these chemicals well away from an open flame.Label the bottle, and store it in a cool place.Transfer to a screw-cap bottle, and mix well.Add 5 ml of absolute ethanol or methanol to the 250 ml mark.Fill a cylinder (250 ml capacity) to the 245 ml mark with the saline solution.Caution: Glacial acetic acid is a corrosive chemical with an irritating vapor, therefore handle it with care in a well-ventilated room.Label the bottle, and store it at room temperature.Add the remaining acid solution, and mix well. ![]()
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